cryopreservation with stemdiff neural progenitor freezing medium Search Results


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KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates

doi: 10.1016/j.celrep.2020.108562

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MSC lines were generated from iPSCs using the STEMdiff Mesenchymal Progenitor Kit (StemCell Technologies, Cat. # 05240) following the manufacturer’s protocol over the course of 21 d. Briefly, iPSCs were dispersed as single cells, plated at ~5 × 10 4 cells/cm 2 , and cultured for 2 d on Matrigel with mTeSR1 medium before the medium was changed to STEMdiff -ACF Mesenchymal Induction Medium.

Techniques: Control, Recombinant, Gentle, Isolation, Gel Extraction, Cell Isolation, Bicinchoninic Acid Protein Assay, Flow Cytometry, Software